Features and Benefits

Highly species-specific. Optimized for use with our range of ECL^™ Detection Reagents. Recommended dilutions to minimize background.

General description

The antibody is prepared by hyper-immunizing goats with purified immunoglobulin fractions from normal rat serum to produce high affinity antibodies. The pooled antiserum is used to produce an immunoglobulin preparation which is then affinity adsorbed to remove cross-reacting antibodies towards mouse, human and rabbit immunoglobulins. These activities are thoroughly depleted to ensure species-specificity. Finally, to select for specific binding to rat IgG, the antibodies are purified using an affinity column of rat IgG. After washing to remove non-specific serum components and low affinity antibodies, the species-specific antibodies are eluted using carefully selected, mild conditions which minimize aggregation and preserve immunological activity, yet which will elute high affinity antibodies.

Legal Information

ECL is a trademark of Cytiva